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Recombinant Protein Expression Systems - BIOT7070
 The Quad

   
   
   
 
Campus: Kensington Campus
 
 
Career: Postgraduate
 
 
Units of Credit: 6
 
 
EFTSL: 0.12500 (more info)
 
 
Indicative Contact Hours per Week: 0
 
 
Fee Band: 2 (more info)
 
 
Further Information: See Class Timetable
 
  

Description

Course topics deal with some basic recombinant DNA techniques, and then heterologous protein expression in prokaryotes and eukaryotes is discussed in greater detail. For prokaryotes, Escherichia coli is the model species chosen and for eukaryotes, the cell systems of yeast and mammalian cells are described. The advantages and disadvantages of the various expression cell systems are outlined. The vectors used for cloning of the protein genes are also described and illustrated. Cloning of genes into the vectors, production and subsequent characterisation of the recombinant protein are also described. These examples are actual biopharmaceutical products currently produced by the biotechnology industry and students are referred to published journal papers throughout the modules.

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© The University of New South Wales (CRICOS Provider No.: 00098G), 2004-2011. The information contained in this Handbook is indicative only. While every effort is made to keep this information up-to-date, the University reserves the right to discontinue or vary arrangements, programs and courses at any time without notice and at its discretion. While the University will try to avoid or minimise any inconvenience, changes may also be made to programs, courses and staff after enrolment. The University may also set limits on the number of students in a course.