Recombinant Protein Expression Systems - BIOT7070

   
   
   
 
Campus: Kensington Campus
 
 
Career: Postgraduate
 
 
Units of Credit: 6
 
 
EFTSL: 0.125 (more info)
 
 
Contact Hours per Week: 0
 
 
Fee Band: 2 (more info)
 
 
Further Information: See Class Timetable
 
 

Description


Course topics deal with some basic recombinant DNA techniques, and then heterologous protein expression in prokaryotes and eukaryotes is discussed in greater detail. For prokaryotes, Escherichia coli is the model species chosen and for eukaryotes, the cell systems of yeast and mammalian cells are described. The advantages and disadvantages of the various expression cell systems are outlined. The vectors used for cloning of the protein genes are also described and illustrated. Cloning of genes into the vectors, production and subsequent characterisation of the recombinant protein are also described. These examples are actual biopharmaceutical products currently produced by the biotechnology industry and students are referred to published journal papers throughout the modules.